Assembly Platforms

A large number of different platforms have been described that enable users to assemble plasmids encoding their own customized TAL repeat arrays. Below we provide links to pages that summarize each of these various methods and provide information about where to find protocols, plasmids, and software needed to practice these approaches.

Solid-Phase Assembly Methods (e.g.-- FLASH, ICA, and others)

Standard Cloning Methods (e.g.—REAL, REAL-Fast, and others)

Golden Gate Assembly Methods

 

DISCLAIMER: Some of the links provided in the web pages above are for resources created by a variety of other different groups. Their provision here does not imply an endorsement of or a recommendation to utilize these methods. The Joung lab has not verified the activities of engineered
TAL effector arrays made on these various other architectures and platforms.
   

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